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COAs & Lab Testing

I should move to China and start being a peptide vendor

Started by member_142 19 replies 9 people Last activity

#2
If a grey-market GH product reports: “94% purity” (usually RP-HPLC peak area) “0.8% dimers + related proteins” (usually SEC) …it STILL tells you almost nothing meaningful about real Somatropin quality. And the reason is very simple: ✅ Those two numbers do NOT add up to 100%. Where is the other 5.2%? If they report 94% monomer + 0.8% dimers/HMW, that leaves: 5.2% unreported, unclassified impurities. That 5.2% can be ANYTHING: oxidised GH deamidated GH clipped/misprocessed GH host-cell proteins host-cell DNA misfolded monomer non-GH protein truncated fragments incorrect disulphide bonds endotoxin contamination analytical artifacts None of this shows up in their “purity” number unless they run the full compendial battery of tests. 🔍 Why this matters Pharma-grade Somatropin has to pass ALL of the following tests: 1. SEC chromatogram (dimers + HMW) Limit ≤4% Grey vial reports 0.8% — fine, but that only covers ONE impurity class. 2. RP-HPLC related proteins Limit ≤6% Grey vial does NOT report this separately. 3. Charged variants (CE-SDS / IEF) Total ≤10%, single impurity ≤2%. Grey vial never reports this. 4. Host-cell protein testing Pharma standard: low ppm. Grey GH: not reported. 5. DNA residual testing Pharma: <10 pg/dose. Grey GH: never reported. 6. Endotoxins Pharma: strict EU/USP limits. Grey GH: very rarely tested, often high. 7. Correct folding & isoform profile Must match the 22 kDa isoform distribution. Grey GH: not tested. 8. Biological potency (IU activity) Grey GH: not measured. ⚠️ What your 94% + 0.8% actually means It means: > “We ran one HPLC test and one SEC test. Here are the peaks we could identify. Everything else? We don’t know.” This is why that “94% purity” number is misleading as hell. A pharma-grade Somatropin batch with 94% monomer has to show exactly what the other 6% is across multiple orthogonal methods. A grey-market Somatropin batch with 94% “purity” has no requirement to tell you what the other 6% is. One is perfectly legal and safe. The other could be a bioactive protein, a misfolded protein, an endotoxin cocktail, or literal E. coli debris.
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#14
Everything is currently normal. Please discuss the corresponding issues in the corresponding topics. If there are scammers, please seek help from the administrator
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#16
MZ Biolabs, Finnrick (offer a free test but you'll find very mixed reviews on their service...), think there's a website called Peptidetest, Chromate There's plenty out there, just need to find reviews on the one you want to use that fits your budget I guess
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#19
not even a book, copy paste chat gpt lol im tired of people assuming they know it all just because they have a little chatbot to tell them what to say
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